Abstract
Objective To investigate the effect of RNA binding protein 6 (RBM6) on the proliferation, migration and apoptosis of laryngeal cancer cells. Methods The expression of RBM6 protein was analyzed by Western blotting in 47 cases of laryngeal cancer and adjacent tissues. RBM6 overexpression cell line and control cell line (RBM6 group and control group) were constructed by RBM6 overexpression lentivirus and control lentivirus respectively. The proliferation of two groups was analyzed by cell counting kit-8 (CCK-8). The migration was analyzed in RBM6 group and control group by scratch test. The apoptosis level in RBM6 group and control group was analyzed by flow cytometry. The growth of tumor cells in vivo in RBM6 group and control group was analyzed by allogeneic tumor transplantation. SPSS 13.0 statistical software was used to analyze the measurement data. Mean±SD was used to express the measurement data, and t-test was used to compare between groups. Results As compared with the level of RBM6 protein in adjacent tissues (1.01±0.23), the expression level of RBM6 protein (0.34±0.12) in laryngeal cancer significantly decreased (t=2.913, P<0.05). As compared with the control group, the cell proliferation ability of RBM6 group significantly decreased (F=3.019, P<0.05). As compared with the control group [(84.18±8.32)%], Scratch healing rate in RBM6 group [(30.11±6.89)%] significantly enhanced (t=4.319, P<0.05). As compared with the control group [(35.61±7.01)%], the apoptosis rate in RBM6 group [(5.23±2.12)%] significantly increased (t=2.192, P<0.05). The results showed that the proliferation rate of cells in the control group was significantly higher than that in the RBM6 group (F=2.908, P<0.05). Conclusion RBM6 can inhibit the proliferation and migration of laryngeal cancer cells, and promote the apoptosis of tumor cells. Key words: RNA binding protein 6; Laryngeal cancer; Proliferation; Migration; Apoptosis
Published Version
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