Effect of rhizobium, VAM and rock phosphate inoculation on growth and productivity and isolation of VAMF spores of Pisum sativum

Tabassum Yaseen

doi:10.19045/bspab.2016.50072

Abstract

Rhizobium, VAM and rock phosphate inoculation has a significant utilization in Pisum sativum.The present study was conducted to study the influence of rock phosphorus fertilization, Vesicular Arbuscular Mycorrhizae (VAM) and rhizobium inoculation on growth and yield parameters of Pisum sativum. The experiment was laid out in randomized complete block design during summer season at the Department of Botany University of Peshawar Pakistan. A composite soil sample was collected for analysis of soil for physical and chemical properties. Rock phosphorus fertilization, application along with AMF and rhizobium inoculation increased the growth parameters significantly. Inoculation under Phosphors deficient soil which have pivotal role in the mycorrhization, growth and productivity. Dual inoculation, Rhizobium and AMF resulted in an increase in grain yield respectively, over no inoculation in Pisum sativum. Maximum growth was observed where AMF along with rhizobium and rock phosphate inoculation were applied. It is concluded that Pisum sativum should preferably be grown with dual inoculation of VAM along with rhizobium and rock phosphate. And vesicular arbuscular mycorrhizal spores in the rhizospheric soils of Pisum sativum at three different stages,vegetative, flowering and fruiting highest spores density recorded at fruiting stage and increased with the age of plant and also effected by the treatment highest in dual inoculation as comapared to single inoculation.

Highlights

Vesicular Arbuscular Mycorrhizae (VAM) and rhizobium inoculation on growth and yield parameters of Pisum sativum
Results of VAM, RHZ, RHZ+VAM, rock phosphate (RP) and VAM+RP inoculation on plant height of Pisum sativum (Tables 1-2 and Fig.1). It is evident from the data VAM+RP and RHZ+VAM enhanced the plant height compared to the control
Our results agree with the findings of [22]. who stated that treatments which included rock phosphate (RP) and seed treatment with phosphate solubilizing bacteria (PSB) and Rhizobium application RP+PSB+Rhizobium recorded higher values of growth parameters including plant height, number of branches per plant, number of seeds per plant and pod length

Summary

Introduction

Vesicular Arbuscular Mycorrhizae (VAM) and rhizobium inoculation on growth and yield parameters of Pisum sativum. Rhizobium and AMF resulted in an increase in grain yield respectively, over no inoculation in Pisum sativum. Arbuscular widespread symbiotic interactions that are mycorrhizal (AM) symbioses are formed by commonly described as the result of coapproximately 80% of vascular plant species evolution between fungi and plants where in all major terrestrial biomes [2,3] Dual inoculation with both microorganisms results in a tripartite mutualistic symbiosis and generally increases plant growth to a greater extent than inoculation with only one [4]. The present studies were conducted to evaluate the beneficial effect of bio-fertilizers (VAM, Rhizobium and Rock phosphate) inoculation alone and in combination on growth, productivity and spore density of

Methods

Results

Conclusion