Abstract
Objective To investigate the impact of different ways and doses of recombinant human interferon α1b(rhIFN-α1b) on antiviral effect on respiratory syncytial virus(RSV)-effected mice and signal pathway of JAK/STAT. Methods Forty-eight mice were divided randomly into 6 groups, negative control group, RSV-infection model group, 12.5, 25.0, 50.0 μg rhIFN-α1b atomization inhalation of intervention group, and 12.5 μg rhIFN-α1b injection of intervention group.After continuous drug therapy for 5 days, the left lung tissues of mice were aseptically dissected in the sixth day.Then it were observed lung tissue pathology changes by optical microscope, and expression of STAT1 and STAT2 protein with laser scanning confocal microscopy. Results It was observed that RSV-infection model mice's lungs had significant inflammatory injury under light microscope.After treatments of rhIFN-α1b, it showed that the mice lung tissue had recovery of inflammation on different degrees.The group of inhaled rhIFN-α1b 50.0 μg was damaged on lightest degree.There were statistically significant differences between each group(all P<0.05). The expression of STAT1 and STAT2 protein in RSV-infection model mice's lungs decreased remarkably under fluorescene microscopy.Treatments of rhIFN-α1b increased the expression of STAT1 and STAT2 inhibited by RSV-infection.The expression of STAT1 and STAT2 protein of the inhaled rhIFN-α1b 50.0 μg group increased significantly.The differences between groups were statistically significant(all P<0.05). Conclusions rhIFN-α1b played an important role in anti-RSV effects, simultaneously, it could improve the activity of JAK/STAT signal pathway inhibited by RSV-infection. The treatment effect of rhIFN-α1b deliveried by atomization inhalation was better than that of intraperitoneal injection. And curative effect is proportional to the atomization inhalation dose within a certain range. Key words: Respiratory syncytial virus; Recombinant human interferon α1b; Lung tissue pathology; Signal transducer and activator of transcription
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