Abstract
Objective: To explore the regulative effect on Survivin of r-HuEPO after traumatic brain injury (TBI) in rats, and understand the neuroprotection mechanisms of r-HuEPO. Methods: Seventy-eight adult Wistar rats were randomly divided into sham operation group (n = 6), TBI group (n = 36) and r-HuEPO group (n = 36). The experimental TBI model was created by Feeney’s method. Samples were obtained after injury for measuring apoptosis of cells by Epics XL Flow Cytometer. Immunochemical method was performed for inspection of expressions of Survivin and NF-κB proteins. Results: Compared to the sham group, the number of apoptotic cells and Survivin, NF-κB immunopositive cells was significantly increased in the injured brain after TBI (P < 0.01). R-HuEPO significantly increased the expression of survivin and NF-κB, but decreased the apoptotic rates. Conclusion: Increased expression of NF-κB by r-HuEPO may play important role in regulating Survivin level, inhibiting neuronal apoptosis in cortex and exerting protective function to neurons.
Highlights
Survivin was one of the new-found genes of the apoptosis inhibitor family
This study is to explore the effect of rHuEPO on NF-κB, Survivin and cell apoptosis after traumatic brain injury (TBI) in rat, in the mean time, illuminate the potential mechanism of neuroprotective effect of Recombinant human erythropoietin (r-HuEPO)
The apoptosis rates of TBI group and r-HuEPO treatment group were significantly higher than samples obtained from sham-operation group. * shows statistic analysis acquires P < 0.01 TBI group compare with sham-operation
Summary
Survivin was one of the new-found genes of the apoptosis inhibitor family. The protein expression of Survivin increased significantly after traumatic brain injury (TBI), it does not express in normal differentiated mature tissue. Recombinant human erythropoietin (r-HuEPO) has neuroprotective effect after traumatic brain injury (TBI), the specific mechanism of it was still unclear [1,2]. This study is to explore the effect of rHuEPO on NF-κB, Survivin and cell apoptosis after TBI in rat, in the mean time, illuminate the potential mechanism of neuroprotective effect of r-HuEPO
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