Effect of quercetin on oxidative stress in 3T3-L1 mature and hypertrophic cells

Abstract

Abstract Purpose: The process of excessive or abnormal accumulation of fat in the body is called obesity, and its prevalence is increasing globally. The imbalance between antioxidants and free radicals, or oxidative stress, can be caused by or result from obesity. Flavonoids with antioxidant potential may help lower the increased oxidative stress associated with obesity. This study aimed to determine how quercetin affected oxidative stress in hypertrophied and mature 3T3-L1 adipocytes. Materials and methods: After differentiating, 3T3-L1 adipocytes were treated with insulin and a glucose-containing medium to become mature (10 days) and hypertrophic (18 days). The cells were subsequently incubated with 80 µM quercetin for 24 and 48 hours. ELISA was used to determine the levels of total antioxidant capacity/total oxidant capacity (TAS/TOS). Using Oil Red O staining, an accumulation of triglycerides in cells was examined. Results: It was shown that the quercetin molecule had a prooxidative effect on hypertrophic adipocytes but did not impact on oxidative stress in mature adipocytes. Conclusion: It is believed that the number and kind of fat cell will determine the appropriate dose and duration of administration for quercetin's antioxidant mechanism of action, which produces numerous beneficial effects.