Abstract
Overweight and obesity are defined as excessive and abnormal fat accumulation that is harmful to health. This study analyzes the effect of different concentrations of the lugol solution (molecular iodine dissolved in potassium iodide) on lipolysis in cultured 3T3-L1-differentiated adipocytes. The mature adipocytes were treated with doses from 1 to 100 µm of lugol for 0.5, 6, and 24 h. The results showed that mature adipocytes exposed to lugol decrease their viability and increase caspase-3 activity with a lethal dose (LD50) of 473 µm. In mature adipocytes, lugol decreased the total intracellular lipid content, being significant at doses of 10 and 100 µm after 6 and 24 h of treatment (P < 0.01), and the accumulation of intracellular triglycerides decreased after 24 h of exposure to lugol (P < 0.05). Lugol treatment significantly increases the release of glycerol to the culture medium (P < 0.05). The levels of adipocyte-specific transcription factors C/EBP-α were downregulated and PPAR-γ upregulated after 30 min with lugol. These results indicate a lipolytic effect of lugol dependent on PPAR-γ and C/EBP-α expression in mature 3T3-L1 adipocytes.
Highlights
Obesity is a chronic disease that affects more than 650 million individuals worldwide and is characterized by excessive increase in adipose tissue due to an imbalance between caloric intake and energy expenditure [1]
It is observed that lugol does not affect viability nor caspase-3 activity in control fibroblasts with doses of 1–100 μm (Figure 1(d))
When examining peroxisome proliferator-activated receptor-c (PPAR-c) expression in mature 3T3-L1 adipocytes treated with 1–100 μm lugol, the results indicate a significant increase in PPAR-c expression at doses of 10 μm (P < 0.01) and 100 μm (P < 0.001) at 30 min of treatment compared to control adipocytes (Figure 3(a))
Summary
Obesity is a chronic disease that affects more than 650 million individuals worldwide and is characterized by excessive increase in adipose tissue due to an imbalance between caloric intake and energy expenditure [1]. Erefore, the present study analyzes the effect of lugol on lipolysis, viability, and apoptosis in mature mouse 3T3-L1 adipocytes and the participation of the transcription factors PPAR-c and C/EBP-α.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
