Effect of Punica granatum peel and Vitis vinifera seeds on Biochemical Indices in DEN induced Hepatocellular Carcinoma in Rats

Abstract

Plants have been the source of traditional medicines for many diseases known to mankind. Many materials which are considered as waste products from plants are reservoir of phytochemicals possessing important biological activities. In the current study the hepatoprotective potential of Punica granatum peel and Vitis vinifera seeds were evaluated in rats against diethylnitrosamine (DEN) induced hepatocellular carcinoma. Rats were divided into four groups. The first group served as normal control group, the second group received DEN at a dose of 200 mg/kg body weight by Single intraperitoneal administration. The third one received DEN as in DEN-treated group and co-treated with 400 mg/kg Punica granatum peel extract (EPGP). The final group also received DEN and co-treated with 400 mg/kg Vitis vinifera seeds extract (EVVS). DEN administration to rats resulted in significantly elevated levels of serum AFP, bilirubin, triglycerides, cholesterol and urea. The levels of plasma total protein, albumin and blood glucose were significantly decreased in DEN treated rats. No significant changes were noted in creatinine levels of DEN induced rats. Co-treatment with the Punica granatum peel and Vitis vinifera seed extracts orally for 12 weeks significantly reversed the DEN induced alterations of above mentioned parameters in the serum. The present study indicates that both the selected extracts exhibits good hepatoprotectivity against diethylnitrosamine induced hepatocellular damage in rats.