Effect of Pulsed Magnetic Field on Red Blood Cell Aggregation : Mobility

Abstract

The disaggregation and displacement of stacked red blood cell (RBC) are known to be dependent on the electric state of hemoglobin and the strength and gradient of external magnetic field. The present study has been investigated the dependence of the change in aggregation and mobility of RBCs on the intensity and time duration of pulsed magnetic field (PMF), in addition to stimulus area. The magnetic field intensities are 0.07, 0.19, and 0.27 T, pulse time of 0.102 ms and pulse repetition rate was 1 Hz. Live blood analysis was used in order to quantitatively estimate the mobility and morphology of RBC exposed to PMF stimulus. The optimal time and intensity of PMF stimulus were 10 minutes and 0.27 T. The difference in the stimulus area did not show much. The continuance of the effect of PMF stimulus was observed to be 2 hours. Also it was verified the movement of the RBCs was accelerated for steady exposure of PMF stimulus. In order to identify a link between disaggregation in rouleau-formed RBCs and PMF stimulus, it is necessary to investigate the correlation between PMF intensity and the stimulus time, the mobility of RBCs, and the number of stacked RBCs.