Abstract
According to concerns about usage of chemical pesticides in the control of cotton boll worm, Helicoverpa armigera (Hübner) (Lep.: Noctuidae), integration of different non-chemical methods, such as the use of enzyme inhibitors, could be a suitable way to control this pest. So, the aim of the current study was to investigate the effect of protein extracts of three bean varieties (Derakhshan, Sadri, Shokoufa) against worm larvae alpha amylase enzyme activity. Sodium chloride (0.1 M) was used to extract proteins from bean varieties. The results showed that protein extracts of different bean varieties have different effects on the insect α-amylase. Derakhshan and Sadri had inhibitory effects on the α-amylase activity, while Shokoufa did not inhibit α-amylase of the insect, considerably. Derakhshan and Sadri at the highest dosage (13 μgml-1) had 70 and 65% inhibitory effect, respectively, whilst at the lowest dose (0.812 μgml-1), inhibited 45 and 32% of the enzyme activity, respectively. In the gel electrophoresis of enzyme without usage of inhibitors, three bands were observed. At the highest concentration of extracts, only one faint band out of the three bands seen in the control was observed and the other three bands completely disappeared. At last, it’s concluded that Derakhshan variety had the greatest effect on the reduction of α-amylase activity.
Published Version
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