Abstract
1. 1. Tyrosylprotein sulfotransferase (TPST) is a key enzyme in the processing of several secretory proteins, including those found in saliva. In this report, the effect of prostaglandins (PG) on TPST activity in submandibular salivary gland was investigated. 2. 2. The results revealed that PGE 2 exhibited TPST stimulatory activity with a 1.5-fold stimulation at 100 μM concentration and a half maximal stimulation at 50 μM. The PGE 2 stimulation was accompanied by an increase in the affinity of TPST towards sulfate acceptor (Km 1.4 μM → 0.12 μM) with little change in V max. 3. 3. The TPST activity was also stimulated by two other major prostaglandins of salivary glands, PGF 2α and 6-Keto-PGF 1α, however to lesser extent, 22 and 23%, respectively. Arachidonic acid, an intermediate prostaglandin precursor, had no effect on TPST activity. 4. 4. The results suggest that prostaglandins and in particular PGE 2 may play a role in the regulation of TPST catalyzed secretory protein tyrosine sulfation in salivary glands.
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