Effect of prostaglandin F 2α dosage and stage of estrous cycle on the estrous response and corpus luteum function in beef heifers

Abstract

Ninety-five normal cyclic crossbred beef heifers were used to determine if the proportions of heifers showing estrus, intervals to estrus and corpus luteum (CL) function were influenced by PGF 2α dosage and (or) the stage of luteal phase when PGF 2α was administered. Heifers were assigned randomly to treatments in a 4 × 3 factorial arrangement. Treatments were 5, 10, 25 or 30 mg PGF 2α injected either in early (5 to 9 d), mid (10 to 14 d) or late (15 to 19 d) stages of the luteal phase. Jugular samples were taken at 0 h and at 8 h-intervals for 48 h and again at 60 h after PGF 2α treatment for progesterone assay. Heifers were observed for estrus continuously for 120 h PGF 2α treatment. The proportion of heifers showing estrus was dependent upon (P<0.05) both dosage of PGF 2α and stage of luteal phase. Heifers given 5 mg of PGF 2α showed estrus only if treated during the late stage, while those given 10 mg of PGF 2α showed a progressive increase of heifers in estrus as stage of luteal phase advanced. The proportion of heifers showing estrus after 25 and 30 mg of PGF 2α increased from 56% for the early stage to 100% for the mid and late stages. Interval to estrus in heifers showing estrus within 120 h after PGF 2α treatment did not differ (P>0.05) among dosages but tended (P=0.10) to be longer in heifers treated during the mid luteal stage (67 h) than in heifers treated in the two other stages (56 h). A greater proportion of heifers (P<0.05) showed estrus by 60 h after PGF 2α when treated during the early and late luteal stages (75.5%) than for heifers treated during the mid luteal stage (30.4%). Patterns of progesterone concentrations were influenced (P=0.08) by the three way interaction of dosage, stage and time. In heifers that showed estrus, rate of decline in progesterone tended (P=0.07) to be slower during the mid luteal stage than during the early and late stages. Progesterone did not drop below 1 ng/ml until 32 h in heifers treated during the mid luteal stage; whereas progesterone dropped below 1 ng/ml by 24 h in heifers treated during the early and late stages. These data may be useful in designing more efficient systems for using PGF 2α or its analogues in estrus synchronization of beef cattle.