Abstract
The influence of the genotype and the number of subcultures on the development of meristem cultures of five mint cultivars and breeding samples – Krasnodarskaya 2, Diana, K37, No. 2.8.14, A2 (Mentha spp.) was studied. At the second stage of micropropagation, the analysis of morphometric parameters of explants during six subcultures of four genotypes showed an increase in the multiplication index at 4-5th passages, while in the sample A2 - at 3-4th passages.
Highlights
Mint is a well-known perennial herbaceous medicinal, essential oil and spicy aromatic plant in the family Lamiaceae
The literature sources contain data regarding the studies related to the development of micropropagation methods for various mint species - Mentha piperita [1,2,3], M. viridis [4], M. canadensis, M. suaveolens, M. longiflora, M. aquatica, M. arvensis, M. x gracilis, M. gracilis, M. spicata [5]
Many important issues of clonal micropropagation were not risen in these works, especially for cultivars and breeding samples of essential oil mint grown in the Crimea
Summary
Mint is a well-known perennial herbaceous medicinal, essential oil and spicy aromatic plant in the family Lamiaceae. The aim of our research was to study the influence of the genotype and the number of subcultures on the development of mint explants at the second stage of micropropagation in vitro. At the 2nd stage of micropropagation, segments of the stem with a node isolated from shoots, which were developed from meristems, were used as explants.
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