Effect of probiotics on the histomorphometry characteristics of Mus musculus Jejunum infected by Salmonella gallinarum

Abstract

Salmonellosis is a disease caused by Salmonella gallinarum, which can cause digestive tract infections. Probiotics are good microorganisms for the host because they can increase the normal bacteria flora in the digestive tract. They can maintain the intestinal mucosal barrier and prevent bacterial adhesion. This study aimed to determine the histomorphometric characteristics of the jejunum from the intestines of mice (Mus musculus) after being infected with S. gallinarum. A total of 20 mice, 4-6 weeks, were divided into four research groups: P1 (probiotics and S. gallinarum infection), P2 (probiotic administration), P3 (S. gallinarum infection), and P4 (control). The probiotics used contain microorganisms such as Lactobacillus casei, Saccharomyces cerevisiae, and Rhodopseudomonas palustris, dissolved in distilled water in a ratio of 1:1000. Probiotics were given orally at 0.5 ml for 7 days. S. gallinarum infection was given orally, with a volume of 0.5 ml (1.5 x 108 CFU/ml). The results showed that the mean score of intestinal lesions differed between groups. The width of the villi, the thickness of the mucosa, and the depth of the intestinal crypts were significantly different. The best result of histology findings was in the group of mice that were induced with probiotics (P2).