Abstract
Early step terpenoid pathway precursors and inhibitors were applied to in vitro root cultures of Aconitum balfourii to examine the effect on probable key enzyme activities, i.e., 1-deoxy-d-xylulose phosphate synthase and 3-hydroxy-3-methyl glutaryl coenzyme A reductase and corresponding change in aconitine content. It was observed that the enzyme activities were stimulated and inhibited as compared to control cultures in the presence of their respective precursors and inhibitors at early stage of incubation; however, aconitine content modulated at later stages. Greater increase in aconitine percentage was observed in the presence of acetyl CoA, a mevalonate pathway precursor then by sodium pyruvate, a non mevalonate pathway precursor. Both pathways inhibitors inhibited the aconitine accumulation, but greater inhibition was observed by mevalonate pathway inhibitor mevinolin (6.2 times) than by non-mevalonate pathway inhibitor fosmidomycin (4.3 times) as compared to control cultures. It was, therefore, concluded that there is possibility of crosstalk between mevalonate and non mevalonate pathways during isopentyl pyrophosphate (IPP) supply for aconitine biosynthesis. However, it seems that the mevalonate pathway plays a greater role in IPP synthesis.
Published Version
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