Effect of Potent Ethyl Acetate Fraction ofStereospermum suaveolensExtract in Streptozotocin-Induced Diabetic Rats

T Balasubramanian,Tapan Kumar Chatterjee,Tamizh Mani,G P Senthilkumar

doi:10.1100/2012/413196

T Balasubramanian, Tapan Kumar Chatterjee + Show 2 more

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https://doi.org/10.1100/2012/413196

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Abstract

To evaluate the antihyperglycemic effect of ethyl acetate fraction of ethanol extract of Stereospermum suaveolens in streptozotocin-(STZ-) induced diabetic rats by acute and subacute models. In this paper, various fractions of ethanol extract of Stereospermum suaveolens were prepared and their effects on blood glucose levels in STZ-induced diabetic rats were studied after a single oral administration (200 mg/kg). Administration of the ethyl acetate fraction at 200 mg/kg once daily for 14 days to STZ-induced diabetic rats was also carried out. The parameters such as the fasting blood glucose, hepatic glycogen content, and pancreatic antioxidant levels were monitored. In the acute study, the ethyl acetate fraction is the most potent in reducing the fasting serum glucose levels of the STZ-induced diabetic rats. The 14-day repeated oral administration of the ethyl acetate fraction significantly reduced the fasting blood glucose and pancreatic TBARS level and significantly increased the liver glycogen, pancreatic superoxide dismutase, and catalase activities as well as reduced glutathione levels. The histopathological studies during the subacute treatment have been shown to ameliorate the STZ-induced histological damage of pancreas. This paper concludes that the ethyl acetate fraction from ethanol extract of Stereospermum suaveolens possesses potent antihyperglycemic and antioxidant properties, thereby substantiating the use of plant in the indigenous system of medicine.

Highlights

Diabetes mellitus is a chronic disorder in the metabolism of carbohydrates, proteins, and fat due to absolute or relative deficiency of insulin secretion with/without varying the degree of insulin resistance [1]
The pet ether, chloroform, ethyl acetate, and aqueous fractions of ethanol extract of Stereospermum suaveolens (EESS) did not show any mortality and toxic manifestations upto the dose of 3200 mg/kg. b.w
Experimental diabetes mellitus was induced by injecting STZ, which is probably due to the destruction of β-cells of islets of langerhans [25, 26] of pancreas leading to high levels of blood glucose in rats

Summary

Introduction

Diabetes mellitus is a chronic disorder in the metabolism of carbohydrates, proteins, and fat due to absolute or relative deficiency of insulin secretion with/without varying the degree of insulin resistance [1]. Medicinal plants used to treat hyperglycemic conditions are of considerable interest for ethnobotanical community as they are recognized to contain valuable medicinal properties in different parts of the plant, and a number of plants have shown varying degree of hypoglycemic and antihyperglycemic activity [2]. Stereospermum suaveolens (Roxb.) DC, family Bignoniaceae, commonly known as Padiri, is a large deciduous tree found throughout the moist parts of India. The decoction of bark and root is used for the treatment of pain, fever, inflammations, asthma, liver disorders, and as a diuretic [3, 4]. In southern India, the bark is used traditionally for the treatment of diabetes [3]. The previous phytochemical studies reported the presence of lapachol [7], sterekunthal B, stereochenols A and B [8, 9] in the bark, and scutellarein [10], stereolensin, dinatin (4,5,7trihydroxyl-6-methoxyflavon), and dinatin-7-glucuroniside [11] in the leaves

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