Abstract
To demonstrate whether potato glycoalkaloids can alter the integrity of membranes of frog embryo, albino frog embryos were incubated with α-chaconine and α-solanine. Di-4-ANEPPS, an electrochromic fluorescent dye, was added to measure embryonic membrane potential. α-Chaconine increased the Di-4-ANEPPS fluorescence up to 1600% of control, α-solanine increased the fluorescence up to 400%, and solanidine had no effect. Increases in fluorescence, when plotted in a concentration-response format, produced EC 50 values near published values for FETAX (frog embryo teratogenicity assay-Xenopus)
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