Effect of Polysaccharides and Water Extract from Fructus tribuli on Growth of Lactobacillus brevis

Zongcheng Miao,Yongming Zhang,Zhongjin Li,Dengwu Wang

doi:10.19026/ajfst.6.3033

Abstract

Effect of polysaccharides and water extract from Fructus tribuli on growth of Lactobacillus brevis was studied by measuring the optical density at 600 nm and pH using commercial MRS broth as the control. The addition (mg/mL) of steroidal saponins was 0.10, 0.20, 0.30, 0.40 and 0.50 and the addition (%, v/v) of water extract was 1, 2, 3, 4 and 5. Results were as follows: The additions of polysaccharides and water extract had a significant promotion on the growth of Lactobacillus brevis. The optimum concentrations of steroidal saponins and water extract were 0.40 mg/mL and 5%, respectively.

Highlights

Tribulus terrestris L. is an annual herb that grows worldwide, especially in the subtropical area of Asia, Africa, Europe, America and Australia (Topia et al, 1994; Kostova et al, 2002; Combarieu et al, 2003)
We found the additions of steroidal saponins and flavonoid had a significant promotion on the growth of Lactobacillus brevis
Preparation of the extract from Fructus tribuli: Fructus tribuli was ground in a high speed disintegrator (Model SF-2000, Shanghai, China), was extracted by distilled water (liquid: solid ratio 14:1, While the temperature (95°C) of the water bath was kept steady, The extraction in a 1.0 l stainless steel boiler in the water bath was stirred with an electric mixing paddle for 1.5 h, obtained the water extract of Fructus tribuli by filtration and centrifugation

Summary

Introduction

Tribulus terrestris L. is an annual herb that grows worldwide, especially in the subtropical area of Asia, Africa, Europe, America and Australia (Topia et al, 1994; Kostova et al, 2002; Combarieu et al, 2003). The occurrence of saponins, polysaccharides, alkaloids, amides, flavonoids and cinammic acid has been reported in Jili (Saleh et al, 1982; Wu et al, 1996; Yan et al, 1996; Wang et al, 1997; Li et al, 1998; Fang et al, 1999; Wu et al, 1999; Kostova et al, 2002). The hot water extract from Fructus tribuli was found to promote the growth of L. acidophilus acidophilus, Lactobacillus bulgaricus and Bifidobacterium bifidum (Shu et al, 2007, 2011a, b). We found the additions of steroidal saponins and flavonoid had a significant promotion on the growth of Lactobacillus brevis. The aim of our research was to study the effect of polysaccharides and water extract from Fructus tribuli on growth of Lactobacillus brevis

Objectives

Methods

Results