Abstract
Ion-exchange electrokinetic chromatography was developed for the separation of analyte ions having identical electrophoretic mobilities in capillary electrophoresis. The separation principle is based on the differential ion-pair formation of the analyte ion with a polymer ion. Polybrene and poly(diallyldimethylammonium chloride) were employed as polymer ions and some isomeric acids as analytes. The dependence of the relative migration velocities of the analytes on the concentration of the polymer ions was studied experimentally, and the results are discussed in comparison with a theoretically derived equation.
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