Effect of Plantain Floret Extract on Gastric Histopathological : Analysis on Wistar Rats Given Ethanol

Abstract

Introduction: Alcohol consumption can cause damage to the gastric mucosa. Floret plantain has antioxidant properties that can prevent damage to stomach cells. This study aims to find out the effect of floret extract of plantain on the histopathological features of Wistar rat’s stomach given ethanol 40%.Methods: This study used the Post-test only group design method. The sample consists of 30 male Wistar rats were divided into 5 groups namely C- was given food and drink standardly, group C+ was given a placebo aquadest 1.8ml/200g/day after 60 minutes given 40% ethanol of 1.8ml/200g/day, group T1, T2, and T3 were given floret extract at a dose (140mg/kg body weight, 280mg/kg body weight and 560mg/kg body weight) and after 60 minutes was given 40% ethanol. The treatment was carried out for 30 days, and then the samples were terminated in order to observe the histopathological microscopically magnification 400x and analyzed with Barthel-Manja criteria. Data were analyzed using Kruskal Wallis and Mann Whitney.Results: The mean of gastric mucosal damage in the C-, C+, T1, T2, and T3 were groups respectively 1.03±0.26, 1.46±0.27, 1.40±0.56, 1.46±0.32, 1.83±0.26. The Kruskal Wallis test results showed significant differences (p=0.02). The Mann Whitney test results showed significant differences in group C- and C+, C+, and T3, while the C+ and T1, T2 showed no significance.Conclusions: Dosing 140mg/kg body weight, 280mg/kg body weight and 560mg/kg body weight were unable to prevent stomach damage to Wistar rats given 40% ethanol.Suggestion : further research and toxicity test.