Effect of phototherapy on blood endothelin and nitric oxide levels: clinical significance in preterm infants

Abstract

Phototherapy may have an adverse effect on the hemodynamics of preterm infants, and endothelin (ET) and nitric oxide (NO) are both the powerful vasoactive substances. This study was designed to observe the effect of phototherapy on blood levels of ET and NO in preterm infants. Sixty-four preterm infants with hyperbilirubinemia requiring phototherapy were studied. Among them, 31 patients were born at 32-36 weeks' gestational age (GA), and 33 patients were < or =32 weeks GA. Control group included 26 full-term infants with hyperbilirubinemia requiring phototherapy. All patients were treated with continuous phototherapy for 24 hours. Blood samples were collected before and after phototherapy. The amount of ET in the blood samples was determined by radioimmunoassay, and NO levels were determined using nitrate reductase. Heart rate, respiratory rate, apnea, and mean arterial blood pressure (MABP) were monitored regularly (defined interval: hourly, 4 hours, etc) during phototherapy. Blood ET levels measured after 24 hours of phototherapy were higher than the pretreatment values, as were blood NO levels measured after 12 hours and 24 hours of phototherapy. Both increases were statistically significant (P<0.05) in the GA< or =32 weeks group. In the GA>32 weeks group, blood NO levels measured after 24 hours of phototherapy were higher than the pretreatment values; these changes were also statistically significant (P<0.05). In the GA<32 weeks group, heart rate increased and the MABP decreased during phototherapy. The changes after 24 hours of phototherapy compared to the pretreatment values were statistically significant. A few episodes of apnea occurred during phototherapy in the GA< or =32 weeks group. This was significantly higher than that in the other two groups. Under phototherapy, blood levels of ET and NO were significantly higher in preterm infants, especially in preterm infants of < or =32 weeks GA.