Abstract
We studied the effects of L-phenylalanine and a-methylphenylalanine on 32P in vitro incorporation into cytoskeletal proteins from cerebral cortex of 17-day-old rats. Slices of cerebral cortex were incubated in the absence or presence of increasing concentrations of l-phenylalanine, α-methylphenylalanine or l-phenylalanine plus α-methylphenylalanine for 1 h. The cytoskeletal fraction obtained from slices was incubated in the presence of the same drugs and the 32P in vitro incorporation into cytoskeletal proteins was measured. Addition of α-methylphenylalanine did not change 32P in vitro incorporation into the cytoskeletal proteins, but phenylalanine decreased the in vitro phosphorylation of β tubulin. Furthermore, addition of l-phenylalanine plus α-methylphenylalanine decreased the in vitro phosphorylation of both 160 kDa neurofilaments and α-tubulin.
Published Version
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