Effect of peroxiredoxin 1 on apoptosis and autophagy of human gastric cancer cells and possible mechanism

Abstract

Objective To investigate the effect and molecular mechanism of peroxiredoxin 1 (PRDX1) in human gastric cancer cell lines. Methods The expression of PRDX1 was detected in gastric cancer cell lines SGC7901, AGS and the normal gastric mucosa cell line GES-1 with real-time quantitative polymerase chain reaction (Real-time PCR). Small interfering RNA to PRDX1 was synthetized for the transfection with lentivirus vector of LV-PRDX1-small interfering RNA (siRNA), which was transfected into AGS. Methyl thiazol tetrazolium (MTT) assay was used to test activity of cells. Flow cytometry (FCM) was used to detect the apoptosis rate and mitochondrial trans-membrane potential. Real-time PCR and Western blotting were used to detect the expression of mRNAs and proteins associated with apoptosis and autophagy. Results The expression of PRDX1 mRNA (1.049±0.124, 1.597±0.103) and proteins (0.873±0.135, 1.083±0.204) in SGC7901, AGS cells was significantly higher than that in GES-1 cells (mRNA: 0.451±0.060, and protein: 0.305±0.022), and the difference was significant (F=200.162, 48.432, P 0.05), and the expression of Beclin-1 and LC-3Ⅱ/LC-3Ⅰ increased significantly (F=30.332, 24.260, P<0.01). Conclusion PRDX1 was up-regulated in gastric cancer cells, and inhibition of PRDX1 can regulate apoptosis and autophagy via regulating apoptosis and autophagy associated genes. Key words: Gastric cancer; Peroxiredoxin1; Autophagy; Target gene