Abstract
Metal nanoparticles are widely used for the delivery and targeting of pharmaceutical, therapeutic and diagnostic agents in cancer therapy in recent years. The multifunctional nanoparticles constructed currently are supposed to show superior effects on cancer cells. This study was conducted to observe the difference between the effect of a biologically important peptide, silver (AgNPs) and gold (AuNPs) nanoparticles and their conjugates on two different cancer cells. Peptide (Boc-L-DP-L-OMe) was acquired from different sources and subjected to conjugation with biosynthesized gold and silver nanoparticles under standard conditions. These conjugates were tested against the colon cancer (HT-29) and breast cancer (MDA MB-231) cell lines. The results clearly depicted the improved activity of nanoparticles in the form of conjugates. Fluorescent dye microscopy and DNA fragmentation assay substantiate the fact that the conjugated nanoparticles cause higher level of disintegration of DNA in cells that consecutively damages and causes apoptosis due to lethality.
Highlights
The existing therapeutics obtained from nanoparticles is site specific or targeted in nature
This study principally aims to conclude the effect of these nanoparticles hybridized to a biologically essential peptide on colon cancer cells
The silver nanoparticles–peptide conjugates were characterized and the analysis confirmed the occurrence of conjugation (Fig. 3)
Summary
The existing therapeutics obtained from nanoparticles is site specific or targeted in nature. Enormous advances in cancer healing and various significant applications associated with drug therapy in medical science field are being observed (Shrivastava et al 2007). The capability of nanoparticles to perform on site-specific areas has been a vital feature in the functionalization studies in nano-biotechnology, which, avoids disturbing healthy tissues and unnecessary side effects of such drugs (Gindy and Prud’homme 2009). The importance of harsh chemical and physical therapy in cancer cure is one more drawback of usual medical remedies. The method, in which the drugs change the healthy tissue in our body alongside the cancer cells and tissues, is caused by their nonspecificity of therapeutic drugs. Heath and Davis stated, the actuality that drugs can be merely administered in later stages in cancer growth
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