Effect of PDTC-induced inhibition of NF-κB expression on cerebrovascular remodeling following subarachnoid hemorrhage

Abstract

Objective To observe the expression of NF-κB in the basilar artery of rabbit model after subarachnoid hemorrhage( SAH) and its effect on cerebrovascular smooth muscle proliferation. Methods Sixteen male New Zealand rabbits were evenly randomized into two groups,ammonium pyrrolidinedithiocarbamate( PDTC) group and SAH group. Double injection of fresh autologous arterial blood into the cisterna magna was used to establish animal models of cerebral vasospasm. In PDTC group PDTC was injected each day into the cisterna magna in the model animals. The animals were sacrificed 7 days after model establishment; the smooth muscle thickness of basilar artery was observed; and expression of NF-κB,proliferating cell nuclear antigen( PCNA) in the basilar artery was examined by immunohistochemistry and Western blotting analysis. Results Compared with SAH group,PDTC group had significantly decreased NF-κB and PCNA protein expression and significantly reduced smooth muscle thickness( P 0. 05). Conclusion PDTC can reduce the proliferation of smooth muscle through inhibiting the activation of NF-κB after SAH in rabbits.