Effects of eritoran on the expressions of IL-1β, TNF-α and IFN-β mRNA in the basilar artery after subarachnoid henmntmge in rabbits

Abstract

[ Objective To investigate the effects of eritoran on the expressions of the inflammatory cytokines interleukin-1β （IL-1β）, tumor necrosis factor-α （TNF-α and interferon-β （IFN-β） mRNA in the basilar artery after subarachnoid hemorrhage （SAH） in rabbits. Methods Atotal of 36 healthy adult male New Zealand white rabbits were randomly allocated into three groups： SAH （n = 12）, normal saline （n = 12） and eritoran （n = 12） groups. A SAH model was induced by injection of autologous arterial blood into cisterna magna twice. An equal amount of cerebrospinal fluid was displaced with the saline in the normal saline group. An equal amount of autologous non-heparinized arterial blood was injected immediate after the replacement of cerebrospinal fluJid in the SAH group. Eritoran 1.5 mg/kg was injected intravenously immediately after the blood injection via the cisterna magna each time in the eritoran group. The food intal（e and neurological deficit were assessed. The expressions of IL-1 β, TNF-α and IFN-β mRNA in the basilar artery were detected by real-time fluorescence quantitative polymerase chain reaction. Results The food intake scores （1.20± 0. 41 vs. 2. 20 ±0. 61 ; t = 53. 073, P = 0. 002）, the neurological deficit scores （1.46±0. 32 vs. 2. 6 ± 0. 08; t = 306. 431, P = 0. 001）, the expressions of IL-1 β （1.22 ±0. 48 vs. 2. 38 ±0. 06, P =0. 000）, TNF-α （1.39 ±0. 07 vs. 3.32 ＋ 0. 21, P =0. 000） and IFN-β （1.51 ±0. 08 vs. 2. 18 ±0. 05, P =0. 000） in Eritoran group were all significantly lower than those in the SAH group. Conclusions Eritoran may downregulate the expressions of inflammatory cytokines IL-1 β, TNF-α and IFN-β mRNA in the basilar artery after SAH in rabbits, inereasing food intake,and improving neurological deficits. Key words: Subarachnoid Hemorrhage; Eritoran; Interleukin-1β; Tumor Necrosis Factor-α; Interferon- β; Toll-Like Receptor 4; Basilar Artery; Disease Models, Animal; Rabbits