Abstract
Effect of Parental Genotypes and Their Reciprocal Crosses on Haploid Plant Production by Anther Culture and Confirmation of Double Haploids by Flow Cytometry in Bread Wheat
Highlights
Bread wheat (Triticum aestivum L.) is one of the most cultivated cereals crop in worldwide including Turkey
These double haploid (DH) lines can be utilized as a recombinant wheat genotype with desired gene combinations
The present study showed that anther-derived DHs lines are superior over the national control cultivar in agreement with Konieczny et al (2012), Barakat et al (2012), and Al-Shaker (2013). 3.4
Summary
Bread wheat (Triticum aestivum L.) is one of the most cultivated cereals crop in worldwide including Turkey. There is need to develop efficient double haploid (DH) technology that accelerates the wheat breeding for desirable agronomic traits including yield (Lantos et al 2013; Asif et al 2014) In this aspect, development of haploid plants from hybrids and followed by chromosome doubling by colchicine, paved the way for accelerating the process of pure wheat lines development (Hansen & Andersen 1998; Jarzina et al 2017). The double haploid technique can be supplemented with modern technology i.e. gene transformation (Chauhan & Khurana 2011), development of a functional marker CAPS (Yue et al 2015), identify the molecular markers (Abd El-Fatah et al 2017) and applied CRISPR system for induction of improve starch quality (Liu et al 2020) for further improvement and releasing of new bread wheat varieties It is well-established fact that anther culture is useful for DHs production in many cultivated crops including wheat. This study aimed to screen in vitro anther culture responses of Atay-85 and Zubkov cultivars and their reciprocal crosses
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