Abstract
Palmitoyl CoA (PaCoA) is an antagonist at human recombinant P2Y1 purine receptors (Coddou et al., 2003), HEK cells, human platelets (Manolopoulos et al., 2008), and rat thoracic aorta. The present study investigated the effect of PaCoA on vasorelaxation evoked by ADP in porcine isolated mesenteric and coronary arteries.In isometric tension recordings, ADP evoked concentration‐dependent relaxations of the pre‐contracted porcine mesenteric and coronary arteries with maximal responses of 40 ± 12 and 95 ± 7% and pEC50 values of 4.26 ± 0.33 and 3.27 ± 0.85 (n = 7–12), respectively. PaCoA (10 μM) had no significant effect on relaxations in the coronary artery but abolished relaxations in the mesenteric artery. Endothelium removal abolished ADP‐induced relaxations in the mesenteric but not coronary artery.The preferential inhibition by PaCoA of ADP‐evoked relaxations in the mesenteric, but not coronary, artery indicates the involvement of distinct mechanisms: endothelial P2Y1 receptors mediate vasorelaxation to ADP in the mesenteric artery while in the coronary artery ADP‐induced relaxation occurs via receptors on the smooth muscle that may involve adenosine release and activation of A2A receptors (Rayment et al., 2006).Coddou et al. (2003). FEBS Lett 536: 145–150Manolopoulos et al. (2008). Platelets 19: 134–145Rayment et al. (2007) FASEB J 21: 577–585Sponsored by the Jordanian government.
Published Version
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