Abstract
Objective: The aim of this study was to evaluate the effect of ozonized oil (OZ) on the oral levels of Candida spp. in patients with denture stomatitis. Material and Methods: In vitro tests were performed to validate antifungal activity and to standardize OZ conditions. Antifungal activity was screened against C. albicans and five non-albicans species (C. tropicalis, C. dubliniensis, C. krusei, C. guilliermondii, and C. parapsilosis). Also, the effects on C. albicans planktonic and biofilm were evaluated. After validation, OZ was included in a therapeutic protocol of denture stomatitis in vivo. Thirty patients used OZ and 20 used sodium bicarbonate (SB) for 14 days. After 7 and 14 days, clinical evaluation, isolation and identification of yeasts were performed. Isolates were identified by phenotypic and genotypic tests. Ozonized oil showed in vitro antifungal activity against all species of Candida. Ozonized oil reduced the number of viable cells in C. albicans biofilms. Oral candidal levels were lower in relation to baseline both after after 14 days of treatment with SB and OZ. Results: A total of 493 Candida spp. isolates was obtained and 80% were identified as C. albicans. Remission of denture stomatitis was observed in all patients after 7 days of treatment in both groups. Conclusion: Within the limits of the study we can conclude that ozonized olive oil can be a new alternative for the control of biofilm in patients with denture stomatitis. KeywordsOzone; Candida; Antifungal Agents; Stomatitis; Denture.
Highlights
Denture stomatitis is a multifactorial condition associated to candidal infection, poor oral hygiene and denture hygiene, trauma, alterations in oral pH, and other systemic conditions [1,2]
This study aimed to evaluate the effect of ozonized olive oil on the oral levels of Candida spp. in patients with denture stomatitis
Ozonized oil was more effective in the eradication of C. albicans biofilm in vitro when compared to sodium bicarbonate
Summary
Denture stomatitis is a multifactorial condition associated to candidal infection, poor oral hygiene and denture hygiene, trauma, alterations in oral pH, and other systemic conditions [1,2]. Dentures predispose to Candida infection in the denture supporting area. Denture stomatitis may be related to materials presenting higher surface roughness [2]. Microbial biofilm accumulated on denture’s surface may create a favorable environment to Candida spp. growth [3] and to the establishment of pathological condition. The structural organization and function of biofilm communities is shown to be less susceptible to antifungal agents [4]. C. albicans is the most frequently isolated species in cases of denture stomatitis (more than 80%) [5]. C. tropicalis, C. glabrata, C. parapsilosis, C. stellatoidea, C. krusei and C. kefyr are found [6]
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