Abstract
To assess and compare the histopathological effects of ozone therapy and/or methylprednisolone (MPS) treatment on regeneration after crush type sciatic nerve injury. Forty Sprague-Dawley male rats were randomly allocated into four groups. Four groups received the following regimens intraperitoneally every day for 14 days after formation of crush type injury on sciatic nerve: Group I: ozone (20mcg/ml); Group II: methylprednisolone (2mg/kg); Group III: ozone (20 mcg/ml) and methylprednisolone (2mg/kg); Group IV: isotonic saline (0.9%). The histomorphological evaluation was made after biopsies were obtained from the sites of injury. Significant differences were noted between groups in terms of degeneration (p=0.019), nerve sheath cell atrophy (p=0.012), intraneural inflammatory cellular infiltration (p=0.002), perineural granulation tissue formation (p=0.019), perineural vascular proliferation (p=0.004), perineural inflammatory cellular infiltration (p<0.001) and inflammation in peripheral tissue (p=0.006). Degeneration was remarkably low in Group III, while no change in nerve sheath cell was noted in Group II. The combined use of methylprednisolone and ozone treatment can have beneficial effects for regeneration after crush type nerve injury.
Highlights
Crush type peripheral nerve injury is a frequent type of nerve injury and insufficient treatment is associated with sensory, motor or autonomic functional deficits
Antioxidants may alleviate the adverse effects of free oxygen radicals on nerve regeneration[3,4]
The present study was performed to evaluate comparatively the histomorphological changes that occur after ozone therapy and/or MPS treatment following crush injury of the sciatic nerve in an experimental rat model
Summary
Crush type peripheral nerve injury is a frequent type of nerve injury and insufficient treatment is associated with sensory, motor or autonomic functional deficits. Regeneration and repair process follow nerve injury can be impaired due to the oxidative stress[1,2]. Antioxidants may alleviate the adverse effects of free oxygen radicals on nerve regeneration[3,4]. Ozone is a powerful oxidant with antibacterial, antifungal and antiviral effects[5]. It enhances the oxygenation of tissues and triggers the secretion of cytokines[6]. It has been used as a therapeutic agent for chronic and diabetic wounds[7]. Ozone is useful for infected and necrotic wounds and poorly oxygenated tissues[6]
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