EFFECT OF OZONE ACTIVATED PLATELET RICH PLASMA ON THE MANAGEMENT OF GRADE II FURCATION INVOLVEMENT (COMPARATIVEEXPERIMENTALSTUDY)

Abstract

INTRODUCTION: Furcation defects are a frequent finding in periodontitis patients. Activated platelets release numerous proteins, among them adhesive glycoproteins and growth factors. Ozone (O3) is a triatomic molecule, consisting of three oxygen atoms, and its application in medicine and dentistry has been indicated for the treatment of different pathologies. AIM OF THE STUDY: The aim of this experimental study was to evaluate the effectiveness of combining Platelet Rich Plasma (PRP) and ozone therapy in the management of surgically created grade II furcation defects. MATERIALS AND METHODS: A split mouth design was carried out using six healthy mongrel dogs. A total of eighteen critical sized grade II furcation defects were surgically created in the third and fourth mandibular premolars, bilaterally. After reflecting a mucoperiosteal flap, the defects in the experimental side were treated with PRP activated by ozone, β-TCP and collagen membranes, whereas, the control side defects were treated with PRP, β-TCP and collagen membrane. The dogs were sacrificed after 4 and 8 weeks. Samples were dissected and prepared for histological evaluation. RESULTS: Histological results showed that PRP activated by ozone led to higher vascularization of the study samples at all time points; this was evident at four weeks in the form of numerous blood vessels entrapping RBCs and at eight weeks by the presence of red bone marrow and blood vessels entrapping RBCs within. Histomorphometry revealed that ozonated PRP accelerated bone regeneration at both time intervals, this acceleration was almost two –fold upon comparing test to controls. CONCLUSION: Activation of PRP by ozone is effective in enhancing angiogenesis and bone formation during periodontal regenerative therapy in grade II critical –sized furcation defectsin dogs