EFFECT OF OXYMATRINE ON TRICLOSAN-INDUCED OVARIAN TOXICITY IN FEMALE RATS.

Abstract

Introduction: Triclosan [TCS] is a broad-spectrum antibacterial xenoestrogencommonly used in cosmetics, soap and various products. Exposure to Triclosancreates a disruption between reactive oxygen species (ROS) and antioxidant defenses.Oxymatrine [OMT] has potent anti-cancer, anti-fibrosis, and anti-oxidant effects. Aimof work: To study the effect of Oxymatrine on Triclosan-induced ovarian toxicity.Material and Methods: The studied rats have been divided into five groups formeasurement of total antioxidant capacity, Caspase-3, the levels of Tumor NecrosisFactor –alpha[TNF- α], Estrogen and Progesterone levels , and Liposaccharide BindingProtein [LBP] gene expression in response to Triclosan-induced ovarian toxicity by realtimePolymerase Chain Reaction [PCR]. Results: Triclolsan [TCS] caused statisticallysignificant reduction in total antioxidant capacity, with statistically significant elevationin TNF-α and Caspase-3 activity compared to the control group. Oxymatrine inducedstatistically significant elevation in total antioxidant capacity, statistically significantdecrease in the level of TNF-α, Caspase-3 activity with increased levels of Estrogenand Progesterone compared to the Triclosan group. Histopathological and electronmicroscope examination revealed vacuolar degeneration and atretic follicles in ratstreated with Triclosan, and significant improvement after Oxymatrine intake. In theTriclolsan + Oxymatrine [TCS+OMT] group, gene expression levels of TNF‑α,Caspase 3, Mitogen-activated protein kinase [MAPK], Nuclear Factor-kappa [NF-KB], and Liposaccharide Binding Protein [LBP] were statistically significant reduced comparedto the Triclolsan group. Conclusion: The use of Oxymatrine [OMT] as anti-oxidant leadto decrease in genetic expression of Tumor Necrosis Factor –alpha [TNF‑α] , Caspase 3,p38-MAPK, [NF-KB], and LBP, in Triclosan-induced ovarian injuries.