Abstract
Objective To study the effects of oxidative stress on apoptosis of bovine retinal capillary pericytes(BRPs) under high glucose condition.Methods BRPs were cultmred for 6 days respectively in control group(5.5 mmol/L)and in high glucose conditions (15,25,35 mmol/L).The sub-cellular structure was observed under electron microscope.Contents of malondialdehyde ( MDA ) and superoxide dismutase ( SOD ) activities were detected respectively.Results The BRPs showed typical apoptotic morphograpy in the high glucose conditions with the apoptosis rates of(28.35 ±0.84)％,(40.43 ±0.93)％ and(50.16 ±0.60)％ in 15 mmol/L,25 mmol/L and 35 mmol/L glucose condition,much higher than that of control group (4.41 ± 0.66) ％ ( F =21.97,P ＜ 0.01 ).The MDA content/SOD activity of BRPs in high glucose groups were higher than those in the control group(P ＜ 0.01 ).The MDA content/ SOD activity was positively correlated with the apoptosis ratc of BRPs( r =0.893,P ＜ 0.01 ).Conclusion Oxidative stress may participated in the apoptosis of bovine retinal capillary pericytes under high glucose condition. Key words: Diabetic retinopathy ; Pericytes; Oxidative stress; Apoptosis
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