Effect of oxidative stress and 3-hydroxytyrosol on DNA methylation levels of miR-9 promoters.

Stefania D’Adamo,Rosa Maria Borzì,Silvia Cetrullo,Flavio Flamigni

doi:10.1111/jcmm.14657

Abstract

Effect of oxidative stress and 3-hydroxytyrosol on DNA methylation levels of miR-9 promoters.

Highlights

Osteoarthritis (OA) is the most common form of arthritis with increasing prevalence
We demonstrated that HT, a polyphenol found in olives and derivatives, can prevent oxidative stress‐induced cell death and autophagy dysfunction by modulating miR‐9 availability and its cognate target SIRT1
Dysregulated levels of miR‐9 in OA patients have been published,[15] and besides SIRT‐1, other targets associated with OA pathogenesis have been reported, including MMP‐1316 and monocyte chemo‐attractant protein 1‐induced protein 1 (MCPIP‐1).[17]

Summary

| INTRODUCTION

Osteoarthritis (OA) is the most common form of arthritis with increasing prevalence It is a multifactorial disease, it is accepted that ageing can induce the onset of OA and has been proposed as the main risk factor of this pathology.[1] The main reactive oxygen species (ROS) detected in chondrocytes are peroxynitrite (ONOO−) and hydrogen peroxide (H2O2), and when their overproduction is not counter‐balanced by an effi‐ cient antioxidant system, the oxidative stress condition occurs that enhances cartilage degeneration and OA.[2] H2O2 supplementation has been shown to elicit oxidative stress in chondrocytes.[3,4] So far, innovative strategies of treatments with no side effects need to be elucidated. Our present work sought to clarify this aspect by studying DNA methylation of the three miR‐9 promoters in response to H2O2 and HT treatments in C‐28/ I2 chondrocytes

| MATERIALS AND METHODS

| DISCUSSION