Abstract
To evaluate the effect of Otostegia persica (O. persica) extract on renal damage induced by ischemia/reperfusion (I/R) in diabetic rats. Forty-eight rats were subjected to right nephrectomy; then, they were allocated into six groups: Sham; Diabetic sham; I/R; Diabetic I/R; I/R+O. persica; Diabetic I/R+O. persica. Diabetes was induced by streptozotocin (200 mg/kg, i.p.). O. persica (300 mg/kg/day, p.o) was administered for 2 weeks. On the 15th day, ischemia was induced in left kidney for 60 min, followed by reperfusion for 24h. Renal functional and biochemical markers were estimated. I/R in both normal and diabetic rats, induced a significant elevation in serum levels of urea and creatinine (p<0.05). Renal I/R induced a significant increase of malondialdehyde, myeloperoxidase and nitric oxide concentrations associated with significant reduction in superoxide dismutase and catalase activities in comparison with the sham group (p<0.05). Diabetic rats that underwent renal I/R exhibited a significant increase in all the studied parameters with a reduction in the antioxidant enzymes as compared to nondiabetic rats (p<0.05). These deleterious effects associated with renal I/R were improved by the treatment with O. persica (p<0.05). Otostegia persica pretreatment protected the renal injury from ischemia-reperfusion in diabetic rats.
Highlights
Diabetes mellitus, causes organ dysfunction and increases the sensitivity of organs to damages[1]
Antioxidants are of interest to biologists and clinicians because they help to protect the bodies of human and animals against damages induced by reactive oxygen species (ROS) generated in some diseases and even in aging process
All rats were subjected to right nephrectomy; they were randomly allocated into six groups each consisting of eight animals: Group 1: Sham: non-diabetic rats were used as normal control group
Summary
Causes organ dysfunction and increases the sensitivity of organs to damages[1]. Ischemic insults are often recurrent in diabetic patients. In the setting of loss of renal blood flow autoregulation that characterizes the post ischemic kidney, renal ischemia/reperfusion (I/R) injury is a major cause of acute renal failure. Renal I/R causes tissue injury via oxygen radicals and oxidative stress caused by an imbalance in the production of reactive oxygen species (ROS) and antioxidant capacity[3]. ROS and nitric oxide (NO) play an important role in mediating cell damage during I/R injury[4]. Neutrophils are the inflammatory cells that produce high levels of ROS during I/R injury. Antioxidants are of interest to biologists and clinicians because they help to protect the bodies of human and animals against damages induced by ROS generated in some diseases and even in aging process. There are many evidences that natural products and their derivatives have efficient antioxidative characteristics, linked to antidiabetic, anti-aging and anti-inflammatory activities[7,8]
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