Abstract
Massive production and disposal of petrochemical derived plastics represent relevant environmental problems. Polyhydroxyalkanoates (PHA) are a renewable alternative that can even be produced from wastes. The production of PHA from acetate using mixed microbial cultures was studied. The effect of two key operational conditions was evaluated, i.e., substrate concentration and cycle length. The effects of these factors on several responses were studied using a surface response methodology. Several reactors were operated under selected conditions for at least 10 solids retention times to ensure stable operation. Results show that conditions providing higher PHA content involve lower biomass productivities. This has a great impact on biomass production costs. Results suggest then that PHA content alone may not be a reasonable criterion for determining optimal conditions for PHB production. If production costs need to be reduced, conditions that provide a lower PHA content in the selection reactor, but a higher biomass productivity may be of interest.
Highlights
If costs need to be reduced, conditions providing a lower content in the need to be reduced, conditions providing a lower PHB content in the selection reactor, but a higher selection reactor, but a higher biomass productivity may be of interest, as long as the reduction of biomass productivity may be of interest, as long as the reduction of PHB content does not involve
Substrate concentration and cycle length proved to have a deep impact on sequential batch bioreactor (SBR) operation for Substrate and cycle lengthBoth proved to have deep impact
Irrespective of the conditions end of the feast and famine stages was shown to have a constant relation, irrespective of the tested
Summary
They are usually non-biodegradable, are produced from non-renewable resources, and have low densities, meaning that they occupy a large volume in municipal landfills. Marine plastics pollution is a growing source of concern. It is mainly caused by single use plastics, which is rapidly changing policies and legislation in many countries around the world [1]. PHA are polyoxoesters of hydroxyalkanoic acids, which are synthesized by some bacteria as intracellular storage compounds [3]. They are biodegradable and can be produced from renewable resources [4]
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