Effect of olive leaf extract on the gene expression for lipid metabolism in ovariectomized rats (814.6)

Abstract

There have not been enough studies regarding estrogen deficiency and lipid metabolism. Even though many postmenopausal women gain excess adipose tissues, the actual mechanism has not been yet fully understood. Thus, we measured mRNA expression after oral administration of olive leaf extract, which has been reported as an antioxidant, hepatoprotective, and hypolipidemic agent, in ovariectomized rats. Ovariectomized rats were administered either 200 or 400 mg/kg body weight of olive leaf extract (n=7 for each group) every day for 10 weeks. Expression of peroxisome proliferator‐activated receptor alpha and acyl‐CoA oxidase mRNA in the liver were significantly higher in the rats administered lower and higher dose of olive leaf extract compared with ovariectomized control. However, mRNA expression of liver carnitine palmitoyltransferase1a was not different among the groups. Expression of peroxisome proliferator activated receptor gamma mRNA in adipose tissue was decreased in the rats with 400 mg/kg body weight of olive leaf extract than ovariectomized control but it was not significantly different among the groups. Adipose triglyceride lipase expression and lipoprotein lipase mRNA did not change compared to ovariectomized control. These results suggest that genes involved in lipid metabolism may be regulated by olive leaf extract administration in ovariectomized rats. Transcription factors for further analyses to expand current results might include sterol regulatory element binding protein 1c and acetyl CoA carboxylase.