Abstract

This study was conducted to determine the best level of noni fruit extract (Morinda citrifoliaL) addition into citrate-yolk extender on the motility of boer goats spermatozoa preserved at 5℃. Semen was collected using artificial vagina once a week from 1 healthy male aged ±7 years,. The treatments were citrateyolk administrated by noni fruit extracts (P0: 0 g, P1: 2 g/100 ml, P2: 4 g/100 ml, P3: 6 g/100 ml, P4: 8 g/100 ml) and stored at 5℃. The parameters were motility spermatozoa percentage. Observations were made at 2, 24, 48, 72 96 hours after refrigeration. The data obtained was analyzed with split plot analysis of variance (ANOVA) follo wed by Duncan test. The average percentage of sperm motility after refrigated for 96 hours in the groups P0; P1; P2; P3; and P4 respectively was 24,20±0,83%; 34,40±4,61%; 40,20±5,71%; 14,64±21,63%; and 9,54±21,33%. The results showed that giving of noni fruit extract significantly affected (p<0,05) on the percentage of motility spermatozoa of boer goat after refrigeration. It was concluded that the administration of 4 g/100 ml noni fruit extract in citrateyolk after stored at 5℃ resulted a greater sperm motility for up to 96 hours.

Highlights

  • Boer goats are a superior breed of goats originating from South Africa, including the type of goat with a fairly high percentage of carcasses reaching 48-60% [1].One of the efforts that is quite effective and efficient in improving genetic quality and population of goats, needs to be supported by the existence of reproductive technology in the form of Artificial Insemination (AI) program.

  • This study was conducted to determine the best level of noni fruit extract (Morinda citrifolia L) addition into citrate-yolk extender on the motility of boer goats spermatozoa preserved at 5°C

  • The data obtained was analyzed with split plot analysis of variance (ANOVA) follo wed by Duncan test

Read more

Summary

Introduction

Boer goats are a superior breed of goats originating from South Africa, including the type of goat with a fairly high percentage of carcasses reaching 48-60% [1].One of the efforts that is quite effective and efficient in improving genetic quality and population of goats, needs to be supported by the existence of reproductive technology in the form of Artificial Insemination (AI) program. As a result spermatozoa will move slowly and even die so they are unable to fertilize an egg[11] In this case, an antioxidant as a "predator" of free radicals is needed to protect spermatozoa in diluents, such as by using fruits extract. Noni fruit can be used as an additional ingredient in the diluent because it contains many antioxidants such as linoleic acid, βcarotene, vitamin C, flavonoids, caprylic acid, xeronin, and proxeronin which can function to ward off free radicals [14,24]. This is consistent with the research of Isnaini and Nuryadi [15], reporting that goat semen diluted with noni fruit extract in concentration 10% in tris diluent was able to maintain the quality of PE goat spermatozoa during cooling process. The authors are interested in further studying about the benefits of giving Noni (Morinda citrifolia L.) extracts in egg yolk citric diluents as an alternative diluents and how much the dose of noni extract that can increase the motility of boer goat spermatozoa stored at 5 C

Place and Time
Methods
Noni FruIt Extraction
Fresh Sement Quality Check
Making Semen Dilution
Quality of Fresh Cement of Boer Goat
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call