Abstract
Abstract Studies were carried out on the effect of the time of nitrogen fertilizer application to tea plants in spring on the quality constituents of new shoots using sand culture in a green house. Fertilizer was applied in spring each week at 300 ppm N starting from 35 d before the first plucking. The contents of total nitrogen, total amino acids, tannin, caffeine, and the composition of the amino acids and amides were determined. Nitrogen application, 5 to 3 weeks before the first plucking, did not affect the content of theanine (γ-glutamyl ethylamide), one of the major constituents of tea quality, and thereafter the content decreased. These results suggested that inorganic nitrogen fertilizer should be supplied until two weeks before the first plucking. 15N-nitrogen labeling experiment was also conducted to study the contribution of 15N-nitrogen in new shoots of tea and the content of theanine, and the results were discussed.
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