Effect of nitrite exposure on haematological status, oxidative stress, immune response and apoptosis in yellow catfish (Pelteobagrus fulvidraco)

Abstract

Nitrite can cause fishes poisoning. This study evaluated the effects of nitrite exposure on haematological status, ion concentration, antioxidant enzyme activity, immune response, cytokine release and apoptosis in yellow catfish. In this study, yellow catfish were exposed to three levels of nitrite (0, 3.00 and 30.00 mg L−1) for 96 h. The results showed that nitrite poisoning could lead to blood deterioration (red blood cell and hemoglobin reduced; white blood cell and methemoglobin elevated), ion imbalance (Na+ and Cl− declined; K+ elevated), oxidative stress (total antioxidant capacity, superoxide dismutase, catalase and glutathione peroxidase activities declined; malondialdehyde accumulation), immunosuppression (lysozyme activity, 50% hemolytic complement, immunoglobulin M, respiratory burst and phagocytic index declined) and cytokines release (TNF, IL 1 and IL 8 elevated). In addition, nitrite poisoning could induce up-regulation of antioxidant enzymes (Cu/Zn-SOD, Mn-SOD, CAT and GPx), cytokines (TNF, IL 1 and IL 8) and apoptosis (P53, Bax, Cytochrome c, Caspase 3, Caspase 9, ERK and JNK) genes transcription. This study suggesting that the nitrite exposure triggers blood deterioration, disrupts the ionic homeostasis, induces oxidative stress, immunosuppression, inflammation and apoptosis in yellow catfish.