Abstract
Objective To construct the nude mice orthotopic model （nude mice liver metastasis model） transplanted with colorectal carcinoma, and to investigate the roles of Kiss-1 gene on survival, growth and metastasis of transplanted tumors. Methods The colorectal carcinoma tissues positive or nega- tive for Kiss-1 gene expression were chosen to construct the nude mice subcutaneous model and orthotopic transplanted model. The growth of tumor and the liver metastasis was observed. Results In the nude mice subcutaneous model, the success rate in the groups positive or negative for Kiss-1 was 37.50% and 100. 00% with the difference being statistically significant （P 〈 0. 05）. In the orthotopic transplanted mod- el, the success rate of the first generation was 41.67% （ 12. 25% for positive Kiss-1, and 75.00% for negative Kiss-1 ）, and that of the second generation was 41.67% （ 56. 25% for positive Kiss-1 and 93.75% for negative Kiss-1 ）. The success rate in Kiss-1 negative groups was higher than in Kiss-1 nega- tive groups （P 〈 0. 05 ）. In the second generation of the orthotopic transplanted model, the incidence of liver metastasis in Kiss-1 positive or negative groups was 33.33% and 80.00% respectively （ P 〈 0. 05 ）. Conclusion The loss of Kiss-1 expression might increase the success rate of the nude mice orthotopic model transplanted with colorectal carcinoma （nude mice liver metastasis model）, suggesting Kiss-1 might restrain the survival, growth and metastasis of the transplanted colorectal carcinomas. Key words: Colorectal carcinoma; Transplanted tumor; Kiss-1
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