Establishment of three orthotopic implantation nude mice models of colorectal cancer metastasis to liver

Abstract

Objective To establish and compare three orthotopic implantation nude mice models of colorectal cancer metastasis to liver. Methods Human SW1116 cell line was inoculated into 5 BALB/ C nude mice. Eighty-four nude mice were divided equally into pouch, fibrin glue, 1×106 cell and 1 × 105 cell microinjection groups. After animals were killed, the metastasis of cancer in situ, the liver, the lung, the spleen, the pancreas, and the mesentery was observed under the microscopy after HE staining. Results The metastasis rate in groups of pouch, fibrin glue, 1×1106 cell and 1×105 cell microinjection was 100% , 100% , 50. 0% and 14. 3% respectively. The liver metastasis tumor in fibrin glue group was bigger than other groups. HE staining indicated that the liver metastasis tumor was derived from colon tumor. Conclusion Among the three orthotopic implantation nude mice models of colorectal cancer metastasis to liver, the fibrin glue method has a high success rate of operation, a high metastasis rate of the liver, and is simple to manipulate. Key words: Colorectal cancer; Orthotopic implantation; liver met astases; Models, animal