Effect of NaHCO3, MgSO4, Sodium Ascorbate, Sodium Glutamate, Phosphate Buffer on Survival of <em>Lactobacillus bulgaricus</em> During Freeze-drying

Abstract

In the present study, the experiments were investigated the effects of different concentrations of cryoprotective agents, such as NaHCO3, MgSO4, sodium ascorbate, sodium glutamate, phosphate buffer, respectively, which used on survival of Lactobacillus bulgaricus during freeze drying. The number of viable cells and survival ratio were measured by the plate count method. The results were as follows: cryoprotective agents played important roles in survival of Lactobacillus bulgaricus during freeze drying. When the relative volume of phosphate buffer was 1.5 (v/v), the number of viable cells was highest, while the survival ratio reached highest, the concentration of sodium ascorbate was 4.5%.