Abstract
Introduction and Aim: In this study, the interaction between Acanthamoeba culbertsoni and Shigella sonnei was analyzed. In addition, the effect of monosaccharides on the cytotoxicity of A. culbertsoni was analyzed through interaction with macrophages. In particular, the effect of monosaccharides on the activity of A. culbertsoni protease was analyzed. Materials and Methods: Association tests were performed for the interaction of A. culbertsoni trophozoites with Shigella sonnei and Escherichia coli DH5 alpha, and the cytotoxicity and proteolytic enzyme activity of A. culbertsoni trophozoites were analyzed. In all these tests, monosaccharides, e.g., mannose, galactose, glucose, and xylose, were pretreated with A. culbertsoni trophozoites. Results: Except for 50 mM and 100 mM mannose, pathogenic S. sonnei by other monosaccharides showed almost 3 times more association than E. coli DH5 alpha. The association of A. culbertsoni trophozoites and S. sonnei by treatment with 100 mM galactose was inhibited by about 102%. The cytotoxicity of A. culbertsoni trophozoites by galactose was inhibited by about 13% and 25% at 50 and 100 mM, respectively. In particular, 50 and 100 mM mannose inhibited A.culbertsoni trophozoites cytotoxicity by 53% and 58%, respectively. A. culbertsoni proteolytic enzymes were strongly induced by 50 mM and 100 mM mannose. Conclusion: The results of this study confirmed that mannose and galactose influenced the interaction of bacteria to A. culbertsoni trophozites.
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