Abstract
Cynoglossus semilaevis shell is a by-product of the Cynoglossus semilaevis, a species of fish mainly distributed along the west coast of Korea. As its skin is very tough and difficult to process, it is not useful as food. For this reason, most of it is discarded except for a small amount that is used as feed, which results in environmental pollution. Considering this, there is a need for research on the development of functional materials using Cynoglossus semilaevis shell. This study focused on the mechanism of in vitro expression function of Cynoglossus semilaevis shell extract (CSE) for skin tissue in human dermal fibroblasts that induced or did not induce wrinkles by UV-B irradiation and aims to use it as a functional material for human skin beauty or wrinkle improvement through extraction and purification. According to the ELISA results using human dermal fibroblast cells, CSE reduced MMP-1 and elastase activity by up to 21.89% and 12.04%, respectively, in a concentration-dependent manner, and increased PIP synthesis by up to 62.24% in a concentration-dependent manner. The RT-PCR test results using mRNA showed the MMP-1, 2, and 3 expression levels were suppressed in the CSE-treated group compared to the UVB-induced group and caused a concentration-dependent increase in TIMP-1 in the CSE-treat group. These results suggest that CSE can maintain and improve skin tissue conditions through MMP/TIMP balancing in human dermal fibroblast cell lines and indicate its potential as a functional material for improving skin diseases and suppressing photo-aging.
Highlights
IntroductionWrinkles are caused by a decrease in hormone levels with age, exposure to UV rays, or physical causes such as repeated muscle use and facial expressions and are characterized by the thinning of the dermis, causing epidermis thinning due to a decrease in the proliferation rate of cells that occupy the basal cell layer of the epidermis [1,2]
Wrinkles are caused by a decrease in hormone levels with age, exposure to UV rays, or physical causes such as repeated muscle use and facial expressions and are characterized by the thinning of the dermis, causing epidermis thinning due to a decrease in the proliferation rate of cells that occupy the basal cell layer of the epidermis [1,2].The dermis is a thick layer of skin beneath the epidermis, which makes up about 90%of our skin
Can maintain and improve skin tissue conditions through matrix metalloproteinases (MMPs)/tissue inhibitors of metalloproteinases (TIMPs) balancing in human dermal fibroblast cell lines and indicate its potential as a functional material for improving skin diseases and suppressing photo-aging
Summary
Wrinkles are caused by a decrease in hormone levels with age, exposure to UV rays, or physical causes such as repeated muscle use and facial expressions and are characterized by the thinning of the dermis, causing epidermis thinning due to a decrease in the proliferation rate of cells that occupy the basal cell layer of the epidermis [1,2]. It mainly consists of connective and elastic fibers composed of hyaluronic acid, collagen, and elastin, which improve skin elasticity [3,4,5]. The collagen and elastin in the dermal layer are strong connective proteins that form the dermal matrix, which prevents our skin from being damaged by external shocks and is known to play a significant role in maintaining skin elasticity. The decomposition of collagen and elastin in human connective tissue directly affects skin elasticity and wrinkle formation [6,7]. MMPs are transcriptionally regulated by cell growth factors, hormones, and cytokines and are essential enzymes for biological processes such as blastogenesis, organogenesis, nerve growth, ovulation, angiogenesis, and tissue absorption and remodeling by degrading
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
