Effect of mineralized recombinant collagen bone modified with BMP-2-derived peptide on biological behavior of bone marrow stromal cells

Abstract

Objective To explore the effect of mineralized recombinant collagen bone modified with BMP-2-derived peptide on the adhesion, proliferation and osteogenic differentiation of bone marrow stromal cells (BMSCs). Methods The mineralized recombinant collagen bone was prepared and the BMP-2-derived peptide was immobilized on the surface of scaffolds through a method of cross-linker. The microcosmic appearance of the scaffolds was observed by scanning electron microscopy. The third generation of BMSCs was seeded onto the scaffolds, using the unmodified mineralized recombinant collagen bone as control. The adhesion and proliferation of BMSCs were analyzed. The alkaline phosphatase activities and calcium content were measured. Results The scanning electron microscopy showed that the surface of scaffolds was porous. X-ray photoelectron spectrometry (XPS) confirmed that the BMP-2-derived peptide was immo-bilized on the surface of scaffolds successfully. The adhesion and differentiation into osteoblasts of the BMSCs were significantly greater than those in the control group (P ＜0.05 ), but there was no sig-nificant difference between the experimental group and the control group in proliferation of the BMSCs (P ＞0.05 ) . Conclusion Since BMP-2-derived peptide can improve the biocompatibility and bioactivity of mineralized recombinant collagen bone, the mineralized recombinant collagen bone modified with BMP-2-derived peptide is a kind of ideal scaffold material for bone tissue engineering. Key words: Tissue engineering; Bone morphogenetic protein; Biomaterial; Stem cells