Study on cytocompatibility and animal implantation test of domestic reticulated vitreous carbon

Abstract

Objective To investigate the possibility of the domestic reticulated vitreous carbon as a kind of scaffold material for bone tissue engineering, the biocompatibility of domestic reticulated vitreous carbon was first successfully tested with bone marrow stromal cells (BMSCs) in vitro and for bone tissue repair in vivo. Methods From June, 2013 to August, 2014, the morphology and proliferation of BMSCs co-cultured with scaffold material in vitro was measured. Differences of measurement were compared with single factor analysis of variance to detect the cytotoxicity of reticulated vitreous carbon. In vivo reticulated vitreous carbon were implanted into the bone defect site and the groin. After 12 weeks, the biocompatibility of reticulated vitreous carbon was observed. Results MTT results showed that after 7d coculture, the survival and proliferation of BMSCs had not been significantly inhibited (P > 0.05). Inverted fluorescence microscope and scanning electron microscope found that newly developed three-dimensional domestic reticulated vitreous carbon could promote adhesion, aggregation and proliferation of BMSCs in vitro. Studies in vivo demonstrate that implanted reticulated vitreous carbon with a high porosity and host bone may produce a stable connection and integration. Conclusion Non-cytotoxic domestic reticulated vitreous carbon can promote the adhesion and proliferation of bone marrow mesenchymal stem cells in vitro and has good bone induction properties in vivo. Key words: Reticulated vitreous carbon; Bone marrow stromal cells; Biocompatibility; Bone defect; Dog