Effect of microvibration on activity of ureteral and portal smooth muscles

Abstract

Spontaneous contractions were elicited by microvibrations (1--80 Hz, 50--400 micron) imposed upon quiescent ureter and portal venous smooth muscles of dogs. The microvibrations increased the rate of spontaneous contractions. A microvibration of larger amplitude gave rise to a more profound increase at each frequency. The acceleration was often accompanied by a reduction in contractile force. The positive chronotropic effect was enhanced by increases in frequency from 20 to 40 Hz and not affected by administration of autonomic blocking agents and tetrodotoxin, but disappeared in a Ca-free environment and reappeared on addition of Ba2+. the simulating action of microvibration was almost proportional to external Ca2+ concentrations ranging from 2.2 to 6.2 mM. The microvibrations were able to elicit spontaneous activity in the preparation, which had been made quiescent by administration of Mn2+. These findings suggested that the positive chronotropic effect may be closely related to an increased Ca2+ influx through the membrane of smooth muscle. Active tension in the ureter in a state of contracture was depressed by imposed microvibrations.