Effect of microRNA-10a on expression of brain natriuretic peptide after myocardial ischemia reperfusion in rats

Abstract

Objective To investigate the effect of microRNA (miRNA, miR)-10a on the expression of brain natriuretic peptide (BNP) in ventricular remodeling after myocardial ischemia reperfusion injury in rats. Methods Forty-five male Wistar rats were randomly divided into sham operation group A (sham operation group, n=15) and myocardial ischemia reperfusion group (n=30). Rats in myocardial ischemia reperfusion group were inflicted by ligating the left anterior descending coronary of rats. After 24 h, 30 survived rats were randomly divided into group B (myocardial ischemia reperfusion group, n=15) and group C (miR-10a group, n=15). Rats in group C were treated with miR-10a agomirs (80 mg/kg body weight) by tail intravenous injection for 4 weeks, and those in group A and group B were treated with negative control reagents. The expression of histone deacetylase 2 (HDAC2) mRNA was detected by Real-time PCR method respectively. The content of BNP was examined by Western blotting. Results After 4 weeks, the relative expression of HDAC2 mRNA in groups A, B and C was 6.58±0.23×10-5, 14.29±2.16×10-5, and 10.11±1.55×10-5 respectively. Left ventricular mass index was 2.10±0.26, 2.61±0.11, and 2.41±0.12 respectively in groups A, B and C. As compared with group B and group C, the expression of HDAC2 mRNA and BNP in the myocardial ischemia reperfusion zone and left ventricular mass index in group A were significantly increased (P< 0.05). The expression of HDAC2 mRNA and BNP in the myocardial ischemia reperfusion zone and left ventricular mass index in group C were significantly higher than those in group B (P< 0.05). Conclusion miR-10a may improve ventricular remodeling after myocardial ischemia reperfusion in rats via inhibiting the expression of HDAC2 mRNA. Key words: MicroRNA-10a; Histone deacetylase 2; Brain natriuretic peptide; Myocardial ischemia; Reperfusion injury; Myocardial remodeling