Effect of microRNA- 193a on the proliferation and migration ability of human lung carcinoma A549 cells by promyosin

Abstract

Objective To investigate the effect and the related mechanism of microRNA (miRNA, miR)- 193a on the proliferation and migration ability of human lung carcinoma A549 cells by promyosin (TPMF). Methods Western blotting was used to select the cell lines with the lowest expression of TPMF in lung cancer cell lines. The TPMF gene was silenced through TPMF Silencing Lentivirus (LV3- TPMF). green fluorescent protein (GFP) fluorescence detector and Western bolting were used to detect the efficiency of gene silence. Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to detect the correlation between miR- 193a and TPMF. Effect of TPMF on the invasion ability of A549 cells was examined by Transwell matrigel invasion assays. Effect of TPMF on the A549 cells proliferation ability was examined by cell clone assays. The correlation between miR- 193a and TPMF was analyzed by Western blotting and RT- PCR. The Effect of TPMF Silencing on Proliferation of Tumor in Nude Mice Survival Rate was detected. Results LV3- TPMF lentivirus could effectively inhibit the expression of TPMF [(88.29±8.62)% vs. (17.82±4.91)%, P=0.010]. Silencing the expression of TPMF could inhibit the invasion ability of human lung carcinoma A549 cells [(351.23±6.92)% vs. (83.26±3.57)%, P=0.023]. Silence of TPMF could inhibit the proliferation of human lung carcinoma A549 cells [(103.82±5.12)% vs. (22.34±1.91)%, P=0.030]. Silence of TPMF could extend the survival rates of nude mice. Conclusion MiR- 193a can inhibit the invasion and proliferation of lung carcinoma A549 cells by regulating the expression of TPMF. Key words: MicroRNA-193a; Lung carcinoma; Transwell; Promyosin; Survival rate