Abstract
Objective To clone the expression vectors of small interfering RNA(siRNA) against FasL gene and to evaluate its inhibition on the FasL mRNA expression in human lung carcinoma A549 cells. Methods According to the criteria of designing siRNA target sequence,two FasL siRNA template DNA sequences were designed and synthesized. The annealed siRNA template was inserted into siRNA expression vector pSilencer-2.0, which was transfected into A549 cells with liposome mediation after PCR identification and DNA sequencing, and the effect of p2.0Si - FasL on FasL mRNA expression of human lung carcinoma A549 cells was detected by quantitative fluorescence PCR. Results The eukaryotic ex-pression vector of short hairpin siRNA against FasL was constructed successfully and FasL mRNA expres-sion was inhibited. Conclusions FasL siRNA eukaryotic expression vector pSi2.0 - FasL constructed successfully could inhibit the expression of FasL mRNA in human lung carcinoma A549 cells. Key words: FasL gene ; RNA interference ; Expression vector; Quantitative fluorescence PCR
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